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. 2023 Jul 12;14(5):2152–2167. doi: 10.1002/jcsm.13292

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© 2023 The Authors. Journal of Cachexia, Sarcopenia and Muscle published by John Wiley & Sons Ltd on behalf of Society on Sarcopenia, Cachexia and Wasting Disorders.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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scRNAseq suggests active myogenesis in cattle at 4 months of age. (A) UMAP graphs show unsupervised clustering of bovine MCs from all breeds and individual breeds identified by scRNAseq. (B) Violin plots show the expression of select DEGs among different MC subpopulations. (C) RNA velocity analysis of MCs. (D) Pseudotime analysis of MCs. (E) Feature plots show the expression of indicated myosin heavy chain genes in myonuclei identified by scRNAseq, and a pie graph shows the muscle fibre type composition revealed by scRNAseq.