DJ‐1 expression is decreased in skeletal muscle from human and mice with muscle atrophy. (A) DJ‐1 immunoblots of young and old aged men (young, 28 and 29 years; old, 65 and 88 years) and women (young, 15 and 26 years; old, 58 and 69 years) muscles lysates. (B) DJ‐1 RNA‐seq expression data from young (n = 15, 25 ± 1 years) and old (n = 21, 78 ± 1 years) human muscles in GSE25941 datasets. (C) The fold change of DJ‐1 protein expression in young (n = 5, 12 months) and old (n = 4, 24 months) male mice muscles. (D) DJ‐1 immunoblots of young (n = 5, 7 months) and old (n = 5, 24 months) male mice muscles lysates and the quantification of blots. (E) DJ‐1 expression in GAS muscle of Flox male mice and immobilized Flox male mice (n = 8). (F) DJ‐1 immunoblots of GAS from mice without/with immobilization and the quantification of blots. Data represented the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, a two‐tailed Student's t‐test was used for statistical analysis.