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. 2023 Jul 19;14(5):2126–2142. doi: 10.1002/jcsm.13290

Figure 6.

Figure 6

FoxO1 pathway is responsible for DJ‐1 ablation‐induced muscle atrophy. (A) Immunoblot analysis of FoxO family and AKT of GAS in immobilization models. (B) Protein level of FoxO1 in cytoplasmic and nuclear fractions of GAS in immobilization mice. (C) Protein level of FoxO1 in cytoplasmic and nuclear fractions of C2C12 myotubes with/without DJ‐1 knockdown. (D, E) Dual‐luciferase assays of Trim63 and Fbxo32 promoter. Luciferase activity was corrected for Renilla luciferase activity and normalized to control group. The putative FoxO site was noted by black circles. Binding site for FoxO1 in the Trim63 promoter was identified at −192/−188. Binding site for FoxO1 in the Fbxo32 promoter was identified at −38/−31. Data represented the mean ± SEM. *P < 0.05, ***P < 0.001, a two‐tailed Student's t‐test was used for statistical analysis. Immo model, immobilized model; Luc, luciferase.