Table 2.
Kinetic parameters of TXNL1 and Trx1. These kinetic parameters in reduction of insulin, l-cysteine and GSSG were calculated from the data shown in Fig. 2 using the Michaelis-Menten fit with nonlinear regression of the GraphPad Prism software.
| TXNL1 | Trx1 | |
|---|---|---|
| Insulin | ||
| kcat (min−1) | 1670 ± 50 | 3237 ± 378 |
| Km (μM) | 16.5 ± 1.3 | 1.4 ± 1.5 |
| kcat/Km (min−1 μM−1) | 102 | 2294 |
| L-cystine | ||
| kcat (min−1) | 1820 ± 86 | 2175.2 ± 80 |
| Km (μM) | 20 ± 2.5 | 0.8 ± 0.5 |
| kcat/Km (min−1 μM−1) | 92 | 2868 |
| GSSG | ||
| kcat (min−1) | 3329 ± 123 | 3102.4 ± 51 |
| Km (μM) | 34 ± 1.7 | 1.0 ± 0.7 |
| kcat/Km (min−1 μM−1) | 98 | 3118 |