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. 2023 Oct 12;14(10):673. doi: 10.1038/s41419-023-06170-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A Work model of Acetaminophen (APAP)-overdose-induced acute liver injury. B The effective efficiency of hepatic AF6 overexpression and MLKL knockout at mRNA levels was examined by RT-PCR. C The circulating levels of serum AST and ALT were measured after APAP injection in C57/BL mice by kit. D HE staining of pathological sections in APAP-overdose induced acute liver injury model and the necrosis area in liver was measured by Photoshop. E Immunoblot of proteins relating to cell death and NF-κB signaling pathway in liver after APAP injection. mRNA transcription changes of (F) inflammatory cytokines genes and (G) NF-κB targeted genes were determined by quantitative RT-PCR. Values were normalized to the mRNA level of the housekeeping gene 36b4 in the respective sample. Data are expressed as mean ± SEM. Pairwise comparisons between groups were conducted using two-tailed non-paired Student’s t tests. ns, not significant (p ≥ 0.05); *p < 0.05; **p < 0.01; ***p < 0.001.