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. 2023 Oct 12;14:6389. doi: 10.1038/s41467-023-42010-1

Fig. 4. Specificity of 1-2C and 3-2E TCR-T cells to combinatorial peptide library screening of KRAS-G12V-9.

Fig. 4

a Amino acid positions of KRAS-G12V-9. (b-s) 9-mer combinatorial peptide library screening with PANC-1 and KRAS-G12V-9 peptide reactive 1-2C or 3-2E TCR-T cells, showing the reactive amino acid residue landscape (KRAS-G12V-9 peptide shown with a black star). Responses of the 1-2C (b–j) and 3-2E (k–s) TCR-T cells to the combinatorial peptide library were presented as indicated. Secreted IFN-γ was analyzed by ELISA with the triplicate wells of co-cultured supernatants of TCR-T cells and PANC-1 cells in the presence of the indicated peptides. The dots represent three technical replicates (n = 1 experiment) of the responses from one representative donor. The columns show means of the three technical replicates. Data are representative of the responses from n = 3 separate donors run independently (Donor S1-S3). The responses were investigated with 1-2C or 3-2E TCR-T cells prepared with T cells from Donor S1. Source data are provided as a Source Data file.