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. 2023 Oct 12;14(10):674. doi: 10.1038/s41419-023-06213-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — FLAG-tagged DARPin F11 or control DARPin (cDP) were immobilized on protein G magnetic beads saturated with anti-FLAG antibody and incubated with primary tissue lysates of mouse skin. As positive controls, pan-p63 and pan-p73 antibodies were immobilized on protein G magnetic beads and also incubated with primary tissue lysates from mouse skin. The pulldowns were analyzed by western blot using the same pan-p63 and pan-p73 antibodies as primary antibodies and a light chain specific secondary antibody (mouse anti-rabbit light chain) to prevent the detection of the heavy chain of the antibodies used for pulldown (as p63 has the same size as the heavy chain). The pulldown experiment was performed in biological triplicates with one exemplary replicate shown.