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. 2023 Sep 29;11:1219218. doi: 10.3389/fcell.2023.1219218

FIGURE 4.

FIGURE 4

ASC spheroids from sRC showed higher secretion of CCL5, CCL10, and VEGF after week 5 of adipogenic induction compared to sSAT and dSAT. Secretion of IL-1α (A), IL-6 (B), IL-8 (C), IL-10 (D), IL-12p70 (E), TNF-α (F), MIP-1α (G), IFN-γ (H), GM-CSF (I), MCP-1 (J) G-CSF (K), bFGF (L), CCL5 (M), PDGF-BB (N), CCL10 (O), CCL11 (P), and E VEGF (Q) of ASC spheroids in weeks 1 and 5 of adipogenic induction from sSAT, sRC, and dSAT. One independent analysis was evaluated in quadruplicate from 162 spheroids of each sample obtained from four independent experiments. Data are expressed as mean ± SD. The ANOVA test evaluated the difference between ASC spheroids from sSAT, sRC, and dSAT within each group: weeks 1 and 5 of adipogenic induction. Dashed lines indicate post-test analyses under both conditions. Solid lines indicate t-test analyses, which were performed to verify the statistical difference between week 1 of adipogenic induction and week 5 of adipogenic induction from sSAT, sRC, and dSAT. Asterisks indicate p values obtained in the post-test and in the t-test (* p < 0.05; **p < 0.001; ***p < 0.001; **** p < 0.0001). SAT, subcutaneous adipose tissue; ASC, stem/stromal cells from adipose tissue; IL, interleukin; IL-6, interleukin-6; IL-8, interleukin-8; IL-10, interleukin-10; IL-12p70, interleukin-12; IL-15, interleukin-15; IFN-γ, interferon-γ; MCP-1, monocyte chemoattractant protein-1; bFGF, basic fibroblast growth factor; VEGF, vascular endothelial growth factor; GM-CSF, granulocyte-macrophage colony-stimulating factor; G-CSF, granulocyte colony-stimulating factor; PDGF-BB, platelet-derived growth factor; and CCL, CC chemokine ligand.