Table 1.

Estimation of mutation rates at GmCPR5 locus following electro-transfection of Soybean protoplasts.

Protoplast samples	Wild-type negative control
	Total	InDel	InDel frequency (%)	Insertion a	Deletion b
T1 (0 V)	1,713,502	164	0.009	5	159
T3 (0 V)	1,207,415	82	0.006	3	79
T5 (0 V)	1,285,534	239	0.018	0	239
Average c	1,402,150 ± 128,435.4	161 ± 37.01	0.011 ± 0.003	2.66 ± 1.18	159 ± 37.71
Cas9 RNP transformants
T1 (700 V)	2,233,489	47,431	2.1	47,238	193
T1 (1,000 V)	1,964,762	72,550	3.7	39,940	32,610
T1 (1,300 V)	2,186,990	176,140	8.1	142,852	33,288
T3 (1,000 V)	736,342	16,884	2.3	14,417	2,467
T3 (1,300 V)	1,363,363	51,246	3.8	47,636	3,610
T5 (1,000 V)	1,812,143	69,095	3.8	38,366	30,729
T5 (1,300 V)	1,709,290	97,417	5.7	60,815	36,602
Average c	1,715,197 ± 183,218	75,823 ± 17,768	4.21 ± 0.72	55,894 ± 14,293	19,928 ± 5,880

RNP, ribonucleoprotein.

^aNumbers of insertions analyzed.

^bNumbers of deletions analyzed.

^cValues of average and standard deviation error.