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. 2023 Jul 20;83(20):3442–3461. doi: 10.1158/0008-5472.CAN-22-1337

Figure 2.

Figure 2. Effects of brain xRT are age dependent. A, Overall survival of animals treated with 4 Gy CT-guided, whole-brain xRT at indicated ages. B and C, Body (B) and brain (C) weight of mice treated with 4 Gy xRT at indicated ages. D, Anxiety, exploration, and motor activity was assessed in animals treated at indicated ages using open field test 30 days after treatment. E, Rearing behavior assessed at 30 days after treatment. F, Anxiety was assessed by comparing time spent in light chamber in animals treated at indicated ages. G, Motor activity was assessed by using the rotarod test in animals treated at indicated age at indicated number of days after treatment. H, Y maze was used to assess L/M in animals treated at indicated ages by comparing time spent in novel arm. I, NORT was used to assess L/M by testing the ability of animals to discriminate between novel and familiar objects. J and K, Depression and despair-like behavior was assessed using the tail suspension and FSTs by timing the duration of animal immobility (freezing) during a 5-minute test period. L, Neurobehavioral composite scoring indicates the significance of differences in neurobehavioral impairments in animals irradiated at indicated ages. M, Histology of brain sections of indicated regions 60 days after 4 Gy xRT in animals treated at post hoc multiple comparisons test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, nonsignificant.

Effects of brain xRT are age dependent. A, Overall survival of animals treated with 4 Gy CT-guided, whole-brain xRT at indicated ages. B and C, Body (B) and brain (C) weight of mice treated with 4 Gy xRT at indicated ages. D, Anxiety, exploration, and motor activity was assessed in animals treated at indicated ages using open field test 30 days after treatment. E, Rearing behavior assessed at 30 days after treatment. F, Anxiety was assessed by comparing time spent in light chamber in animals treated at indicated ages. G, Motor activity was assessed by using the rotarod test in animals treated at indicated age at indicated number of days after treatment. H, Y maze was used to assess L/M in animals treated at indicated ages by comparing time spent in novel arm. I, NORT was used to assess L/M by testing the ability of animals to discriminate between novel and familiar objects. J and K, Depression and despair-like behavior was assessed using the tail suspension and FSTs by timing the duration of animal immobility (freezing) during a 5-minute test period. L, Neurobehavioral composite scoring indicates the significance of differences in neurobehavioral impairments in animals irradiated at indicated ages. M, Histology of brain sections of indicated regions 60 days after 4 Gy xRT in animals treated at post hoc multiple comparisons test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, nonsignificant.