Figure 4.

Digital sorting analysis carried out on bulk CD34⁺ MNC transcriptome from MDS^RS+ cases. A, Digital sorting analysis, gated for HSPCs. Mature cells were gated out to focus only on HSPC compartment (results including mature cells are shown in Supplementary Fig. S13); CMP, common myeloid progenitors; GMP, granulocyte–monocyte progenitors; HSC, hematopoietic stem cells; MLP, multi-lymphoid progenitors; MPP, multipotent progenitors; MEP, megakaryocyte-erythroid progenitors). Cases (x-axis) are sorted by decreasing estimated MEP abundance, showing enriched IMP signature in cases with MEP depletion on right side of the plots. B, Relative distribution of estimated MEP in the three transcriptomic groups and (NBM). C, Scatter plot comparing results from multiparameter flow (x-axis) and in silico cytometry (y-axis). MEP, GMP, and HSC were quantified in the CD34⁺ BM MNCs by means of the two techniques, showing a highly concordance [correlation line and 95% confidence interval (CI) are represented in dark and light gray, respectively, Pearson correlation coefficient = 0.9, P < 0.001].