Fig. 10.

Histological analysis of high-magnification ( × 600) images of alkaline phosphatase (ALP)-stained sections of the critical-size bone defect in the mRCP, CA and control groups after 4, 8, and 12 weeks. ALP-stained sections in the (A–C) mRCP, (D–F) CA, and (G–I) control groups. Scale bars represent 10 μm. Arrows indicate osteoblasts. (J) The ALP positive area was calculated at 4, 8, and 12 weeks after implantation with bone graft materials using ImageJ software. ∗P < 0.05. Asterisks indicate CA granules. The columns and error bars in the graph represent the mean and SD (n = 3), respectively.