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Growth curve for recombinant S. mutans as a function of time and bioluminescence. The growth of S. mutans cells in BHI broth containing 10 μg of chloramphenicol per ml was followed with a spectrophotometer (OD600; curve) and a manual luminometer (relative light units [RLU]; bars) after the addition of d-luciferin at 200 μM and pH 5.0. The values are means of two independent experiments. The variation between the measurements was less than 10%.
