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Light emission as a function of time and pH. The bioluminescence (in relative light units [RLU]) of logarithmic-phase S. mutans cells was measured at pH 4.5 (•), 5.0 (∗), 5.5 (×), 6.0 (▴), 6.5 (■), and 7.0 (⧫) after the addition of 200 μM d-luciferin (a). (b) Peak relative light units as a function of pH. For details, see Materials and Methods. The values are the means of two independent measurements. The variation between the measurements was less than 10%.
