TABLE 3.
Effects of d-luciferin concentrations on bioluminescence of S. mutans cells at pH 5.5, 6.0, and 6.5a
| pH | RLU in the presence of the following d-luciferin concn (μM)b:
|
|||||
|---|---|---|---|---|---|---|
| 5.0 | 12.5 | 25 | 50 | 125 | 250 | |
| 5.5 | 8.8 | 11.2 | 11.8 | 12.8 | 13.3 | 11.9 |
| 6.0 | 6.3 | 10.4 | 13.1 | 15.4 | 17.1 | 17.8 |
| 6.5 | 3.2 | 6.3 | 9.2 | 12.3 | 16.6 | 16.3 |
a
Different amounts of d-luciferin were added to the S. mutans cells, and the bioluminescence was measured.
b
The results are the means of two independent measurements. Variation between the measurements was less than 10%.