Table 3.

PCR primers used in this study.

Gene	Primer	Sequence	Annealing T	Source
COI	LCO1490	5’ GGTCAACAAATCATAAAGATATTGG	43–45 °C	Folmer et al. 1994
	HCO2198	5’ TAAACTTCAGGGTGACCAAAAAATCA		Folmer et al. 1994
COI	CO1LF	5’ TTTCAACAAATCATAAAGATAT	43–45 °C	Norenburg, unpublished
	CO1DR	5’ GAGAAATAATACCAAAACCAGG		Norenburg, unpublished
16S rRNA	16SARL	5’ CGCCTGTTTATCAAAAACAT	45–50 °C	Palumbi et al. 1991
	16SBRH	5’ CCGGTCTGAACTCAGATCACGT		Palumbi et al. 1991
16S rRNA	16SAF	5’ TCGTCTGTTTATCAAAAACATAGY	45–50 °C	Norenburg unpublished
	16SKR	5’ AATAGATAGAAACCAACCTGGC		Norenburg unpublished
18S rRNA	18SF1	5’ CTGGTGCCAGCAGCCGCGGYAA	55 °C	Machida and Knowlton 2012
	18SRC2	5’ TCCGTCAATTYCTTTAAGTT		Machida and Knowlton 2012
28S rRNA	LSU3	5’ TCCTGAGGGAAACTTCGG	55 °C	Littlewood 1994
	LSU5	5’ ACCCGCTGAAYTTAAGCA		Littlewood 1994