FIG. 3.

In vivo protection of mice from GalN-LPS- and GalN–TNF-α-induced liver injury due to novobiocin pretreatment. BALB/c mice received 700 mg of GalN per kg plus 10 μg of LPS per kg or 700 mg of GalN per kg plus 7.5 μg of TNF-α per kg 1 h after pretreatment with 100 mg of novobiocin per kg. Control animals were pretreated with saline instead of novobiocin. At 8 h after challenge blood was withdrawn for an alanine aminotransferase level determination. The plasma TNF-α level was determined 60 min following LPS treatment. Data are expressed as means ± standard errors of the means (n = 6 for GalN-LPS or n = 3 for GalN–TNF-α). ∗, P < 0.05 versus saline-pretreated mice.