FIG. 4.
Effect of novobiocin on CD14 expression. PBMCs (4 × 106/ml) were incubated in the presence or absence of novobiocin (0.5 mM) for 10 min before LPS (100 ng/ml) was added for 6 h. The cells were analyzed by flow cytometry with MAbs against CD14. The fluorescence of labelled monocytes and lymphocytes was recorded after gating of the cells by using their forward and side scatter properties. Curves represent isotype-matched controls for cells treated with LPS in the presence (B) or absence (A) of novobiocin. Data represent the results of one of three experiments.