TABLE 3.
Effect of novobiocin on antibody-induced modulation of CD14 expression on human monocytesa
| Time (min) | Mean fluorescence (% of that of control) in the
presence of the following:
|
|
|---|---|---|
| LPS at 100 ng/ml | Novobiocin at 0.5 mM + LPS at 100 ng/ml | |
| 0 | 100 | 100 |
| 120 | 100 | 100 |
| 240 | 97 | 90 |
| 360 | 88 | 22 |
a
Monocytes (4 × 106/ml) were stimulated with LPS (100 ng/ml) in the absence or presence of 0.5 mM novobiocin. After 10 min the cells were incubated with FITC-labelled anti-CD14 MAb at 4°C for 20 min. Then cells were washed and incubated in culture medium at 37°C. After the indicated times cells were analyzed by FACS analysis. The mean fluorescence of the cells at time zero was taken as 100% (control). Data from one of three experiments are shown.