TABLE 4.
Effect of novobiocin on shedding of CD14a
| Time (min) | Soluble CD14 concn
(ng/ml) in the presence of the following:
|
|
|---|---|---|
| LPS at 100 ng/ml | Novobiocin at 0.5 mM + LPS at 100 ng/ml | |
| 0 | 0 | 0 |
| 120 | 0 | 0 |
| 240 | 5.5 ± 0.1 | 9.0 ± 1.1 |
| 360 | 25.0 ± 3.0 | 50.5 ± 8.2 |
a
PBMCs (4 × 106/ml) were incubated in the presence or absence of LPS (100 ng/ml) for 6 h. Novobiocin was added 10 min before addition of LPS for 6 h. Supernatants were harvested and analyzed for soluble CD14 by ELISA. Values are means ± standard deviations of one of two experiments.