Fig. 3.
Responses of TE671 nAChRs with membrane potential dye are consistent across a 96-well plate. Scatter plots of the fluorescent response of TE671 cells from wells of a 96-well plate pre-incubated with concentrations of α-BgTx that either give maximal inhibition (30 nM, MIN, red), a middle level of inhibition (3 nM, MID, yellow), or no inhibition (none, MAX, blue), followed by the addition of 10 µM ACh. Each condition was applied to alternating columns of three separate plates (n = 3). Each row of plots contains data generated from one plate. Each plate was recorded on a different day with cells of a different passage number and the assigned MIN, MID, and MAX columns were changed on each plate. Each data point is the resulting RFU value after calculating Fmax-Fbaseline and are plotted by column (A) or by row (B). (C) Summary table of the RFU mean, standard deviation (SD) and coefficient of variance (CV) for each control (MIN, MID, MAX) for each plate. The final column presents the RFU mean once normalised to the MIN and MAX controls.