Table 3.

Delivery of CRISPR-Cas9 RNP by EVs

Method	EV source	Target organ/cells	Target gene	Loading method	Reference
Pre-isolation	Expi293F-derived EVs	HEK293T	fluorescent protein	transfection (PEI Max)	Osteikoetxea et al.76
	HEK293T-derived EVs	HEK293T-eGFP cells	fluorescent protein	transfection (calcium phosphate)	Whitley et al.77
	HEK293T cells derived EVs	U2OS GFP-negative cells	fluorescent protein	Fugene 6 (Promega) and TurboFect	Wang et al.78
	HEK293T cells derived EVs	HEK293T-derived HBB-IL2RG EGFP reporter cells	HBB/IL2RG and DMD exon 53	Fugene HD (Promega)/polyethylenimine	Yao et al.75
	293T cells derived Gectosomes	MEF cells	PINK1 or PCSK9	transfection (polyethylenimine)	Zhang et al.135
	HEK 293T cell-derived exosome	A549stop-DsRed reporter cell line	stop-DsRed	transfection (Lipofectamine 2000)	Ye et al.136
	Hepatic stellate cells-derived exosome	Liver	PUMA, CcnE1,(KAT5	electroporation	Wan et al.137
	HEK293T cells	Skeletal muscle cells	DMD	transfection (Lipofectamine 2000)	Gee et al.84
	MSCs-derived exosome	HEK293T-EGFP cells	fluorescent protein	Lipofection (chemical) and electroporation (physical)	Hazrati et al.138
	Tumor cells	Liver	fluorescent protein	transfection (Lipofectamine 2000)	Ye et al.86
	HEK293T	HeLa cell	fluorescent protein	transfection (Endofectin Max reagent)	Strohmeier et al.87
Post-isolation	HEK293T cells-derived	Muscle	DMD	transfection (Lipofectamine 2000)	Watanabe et al.85
	C2C12 cells	Muscle	miR-29b	transfection (PEI MAX)	Chen et al.89
	HEK293T cells-derived	Tumor	WNT10B	sonication/freeze-thaw cycles	Zhuang et al.139
	Human or mouse serum	Mouse Muscle	DMD	protein transfectant	Majeau et al.88