FIG. 6.

Ciprofloxacin does not influence the half-lives of immediate-early gene transcripts in activated PBLs. Northern blot results are shown for c-myc, c-fos, c-jun, junB, and fra-1 mRNAs. For comparison, IL-2 mRNA was included. Scanning densitometry data are shown below the specific mRNA bands and were obtained by dividing all values by the corresponding values for β-actin mRNA. Control cell cultures (without any addition of drugs) were set to 100 arbitrary units for the different mRNAs. Lane numbers are indicated at the bottom. Human PBLs were stimulated with PHA (4 μg/ml) for 6 h, at which time ciprofloxacin (80 μg/ml) and/or actinomycin D (Act D [10 μg/ml]) was added. mRNAs were analyzed at 0, 2, and 4 h of additional lymphocyte incubation as described in the legend to Fig. 1. The data presented are representative of three independent experiments including different blood donors.