TABLE 2.

Susceptibilities of constructed mutants to various antibiotics

Strain	Relevant genotypea	MIC (μg/ml) ofb:
		SPFX	CPFX	NFLX	LVFX	CZOP	CPR	CAZ	CPZ	CBPC	AZT	TC	CP
PAO1	Prototroph	0.39	0.10	0.39	0.39	0.39	1.56	1.56	6.25	12.5	1.56	6.25	12.5
OCR1	nalB	1.56	0.78	3.13	3.13	1.56	6.25	6.25	25	100	12.5	25	100
KG2212	PAO1 mexR::res-Ω	1.56	0.39	1.56	1.56	0.78	3.13	3.13	12.5	50	6.25	12.5	50
KG2239	PAO1ΔRABM	0.05	0.012	0.05	0.05	0.10	0.39	0.20	0.39	0.39	0.10	0.20	0.78
KG3052	Type-A nfxB	6.25	1.56	6.25	3.13	3.13	6.25	0.78	3.13	6.25	0.78	12.5	25
KG2217	KG3052 mexR::res-Ω	6.25	1.56	6.25	3.13	3.13	6.25	3.13	12.5	50	6.25	25	50
KG2240	KG3052ΔRABM	6.25	1.56	6.25	3.13	3.13	6.25	0.39	0.78	0.39	0.10	3.13	12.5
KG3056	Type-B nfxB	12.5	3.13	12.5	6.25	6.25	12.5	0.78	3.13	1.56	0.39	25	50
KG2236	KG3056 mexR::res-Ω	12.5	3.13	12.5	6.25	6.25	12.5	3.13	25	50	6.25	25	50
KG2259	KG3056ΔRABM	12.5	3.13	12.5	6.25	6.25	12.5	0.78	1.56	0.39	0.10	25	50

^aΔRABM, deletion of mexRAB-oprM operon. 

^bAbbreviations: SPFX, sparfloxacin; CPFX, ciprofloxacin; NFLX, norfloxacin; LVFX, levofloxacin; CZOP, cefozopran; CPR, cefpirome; CAZ, ceftazidime; CPZ, cefoperazone; CBPC, carbenicillin; AZT, aztreonam; TC, tetracycline; and CP, chloramphenicol.