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. 2023 Oct 13;22:208. doi: 10.1186/s12934-023-02217-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Plasmid curing using the clmPheS as a counterselection. (A) Transformation efficiencies of the shuttle vector pEZ15Asp and the clmPheS-expressing plasmid pSsp-RFP. Three replicates were performed for each DNA sample. (B) Growth curve measurements of the pEZ15Asp and pSsp-RFP transformants. Three replicates were performed for the experiment. (C) Examination of the counterselection effect of 4-CP on plasmid curing from the pSsp-RFP transformant. (D) Detection of RFP signal in cells of the pEZ15Asp transformant, and that of the pSsp-RFP transformant before and after 4-CP counterselection