Extended Data 4. Temporal dynamics of IT^PlxnD1 and PT^Fezf2 within parietofrontal and frontolateral networks centered to lick and hand lift onset.

a. Difference between IT^PlxnD1 and PT^Fezf2 mean activity map from Fig 3a,d. Only significantly different pixels are displayed (two-sided Wilcoxon rank sum test with p-value adjusted by FDR = 0.05, n = 24 maps from IT^PlxnD1 and 23 maps from PT^Fezf2). Blue pixels indicate values significantly larger in IT^PlxnD1 compared to PT^Fezf2 and vice versa for green pixels.

b. Distribution of Pearson’s correlation coefficients within IT^PlxnD1 (blue), PT^Fezf2 (green) and between IT^PlxnD1 & PT^Fezf2 (blue green) mean feeding sequence activity maps (n = 253 pairs within IT^PlxnD1, 276 pairs within PT^Fezf2 and 522 pairs between IT^PlxnD1 & PT^Fezf2).

c. Distribution of IT^PlxnD1 (blue) and PT^Fezf2 (green) mean feeding sequence activity maps projected to the subspace spanned by the top two principal components (n = 24 maps from IT^PlxnD1 and 23 maps from PT^Fezf2).

d. Single trial heatmaps and mean activity of PT^Fezf2 and IT^PlxnD1 from parietal, frontal, FLA and FLP centered to lick (top) and handlift onset (bottom, IT^PlxnD1 - 23 sessions from 6 mice, PT^Fezf2 - 24 sessions from 5 mice, shaded region indicates ±2 s.e.m). ***p<0.0005. For box plots, central mark indicates median, bottom and top edges indicate 25^th and 75^th percentiles and the whiskers extend to extreme points excluding outliers (1.5 times more or less than the interquartile range). All statistics in Supplementary table 1.