TABLE 1.
Oligonucleotides used in this study
| Designation | Sequence (5′ to 3′)a | Positionsb | Strandc | Purposed | Reference or source |
|---|---|---|---|---|---|
| DP1 | ACGGCGGCCGTAACTATA | 1985–2002 | + | Commonly used for SDM, sequencing | 30 |
| ZGE23 | ACAGGCCAGTTAGCTA | 2292–2277 | − | Commonly used for SDM, sequencing | 30 |
| GW1 | GACGGGAAGACCCCGTGGA | 2137–2155 | + | SDM (A2142G) | This work |
| GW11 | GGTCTTCCCGTCTTGCCGC | 2148–2130 | − | SDM (A2142G) | This work |
| GW2 | GACGGCAAGACCCCGTGGA | 2137–2155 | + | SDM (A2142C) | This work |
| GW12 | GGTCTTGCCGTCTTGCCGC | 2148–2130 | − | SDM (A2142C) | This work |
| GW3 | GACGGTAAGACCCCGTGGA | 2137–2155 | + | SDM (A2142T) | This work |
| GW13 | GGTCTTACCGTCTTGCCGC | 2148–2130 | − | SDM (A2142T) | This work |
| GW4 | GACGGAGAGACCCCGTGGA | 2137–2155 | + | SDM (A2143G) | This work |
| GW14 | GGTCTCTCCGTCTTGCCGC | 2148–2130 | − | SDM (A2143G) | This work |
| GW5 | GACGGACAGACCCCGTGGA | 2137–2155 | + | SDM (A2143C) | This work |
| GW15 | GGTCTGTCCGTCTTGCCGC | 2148–2130 | − | SDM (A2143C) | This work |
| GW6 | GACGGATAGACCCCGTGGA | 2137–2155 | + | SDM (A2143T) | This work |
| GW16 | GGTCTATCCGTCTTGCCGC | 2148–2130 | − | SDM (A2143T) | This work |
| GW7 | AGACGAAAAGACCCCGTGG | 2136–2154 | + | SDM (G2141A) | This work |
| GW17 | GTCTTTTCGTCTTGCCGCG | 2147–2129 | − | SDM (G2141A) | This work |
a
The bases different from those in the wild-type 23S rRNA gene sequence are underlined.
b
Positions refer to the new numbering scheme for the H. pylori 23S rRNA (30).
c
The plus (+) strand is equivalent to the rRNA sequence, and the minus (−) strand is the complementary one.
d
SDM, site-directed mutagenesis.