Figure 2.
High-content analysis in classical CSA. (A) Left two images show the assay plates and the merged tilescans, scanned at 10× magnification. The right panel shows the raw scanned images of two clones and the masked images used to distinguish cell nuclei generated using CloneFinder 5.0. (B) Clonogenicity analysis after manual and automatic counting of clones for four different cell lines exposed to increasing doses of X-rays. N = 3. (C) Ratio of survival fractions from B. N > 3. ns: not significant.
