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. 2023 Sep 28;15(19):4764. doi: 10.3390/cancers15194764

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Overview of the pan-cancer cohort and study design. (A) Cohort of investigated cancer patients (n = 1800) distributed across eight different organs (each color highlights tissue origin of the cancer). (B) Distribution of patient samples (n = 1800) across fifteen different cancer subtypes. (C) Overview of analytical workflow, which includes library generation (top row) and flow of patient samples with spike in SIS-PrESTs (bottom row) prior to chromatography extraction and data analysis (middle).