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Surface plasmon resonance analysis SERCA2 binding to Arg11. Biotin-Arg11 was immobilized on SA chips and exposed to recombinant 12xHis-SERCA2111–253 protein with stepwise increases in concentration. For the illustrated example, the employed concentrations ranged from 0.7–200 nM. Experimental data are displayed in pink, with fitting by the Langmuir mathematical model superimposed in black. n = 4.
