TABLE 1.
Bacterial strains used in this study
| Strain | Organism | Plasmid | Characteristics | pI of β-lactamase(s) | Source or reference |
|---|---|---|---|---|---|
| 28 | S. typhimurium | pCLL3425 | Clinical isolate | 8.8 | This study |
| 34 | S. typhimurium | pCLL3422 + pCLL3425 | Clinical isolate | 7.6, 8.8 | This study |
| DH5α | E. coli | E. coli K-12 cloning strain | None | 14 | |
| F φ80dlacZΔM15 Δ(lacZYA-argF) U169 recAI endAI hsdR17 (rK− mK+) supE44 thiI gyrI relAI | |||||
| DH5α | E. coli | pCLL2300 | Kmr cloning vector | None | 24 |
| DH5α | E. coli | pCLL3422 + pCLL2300 | Transconjugant of S. typhimurium 34 | 7.6 | This study |
| DH5α | E. coli | pCLL3425 | Transformant of S. typhimurium 34 | 8.8 | This study |
| DH5α | E. coli | pCLL3417 | 7-kb BamHI fragment containing blaCTX-M-5 in pCLL2300 | 8.8 | This study |