FIG. 6.

Efficacy of daily oral prophylactic use of MK-991 as measured by hybridization with a P. carinii-specific DNA probe. Total genomic DNA prepared from lungs of individual animals was immobilized on a nylon membrane in three dilutions, 25 i.e., (column i), 2.5 (column ii), and 0.25 (column iii) μg. (A) P. carinii-specific hybridization in nonmedicated, infected control mice (n = 15); (B, C, D, and E) results from mice treated with 0.78 (n = 11), 1.56 (n = 20), 3.13 (n = 20), and 6.25 (n = 20) mg of MK-991/kg, respectively. The ED₉₀ for prevention of P. carinii trophozoites was estimated to be 1.1 mg/kg.