Table 2.

Cellomics analysis.

		CTRL	PA:OA (1:2)	PA:OA + α-lipoico 1 µM	PA:OA + α-lipoico 5 µM
Steatotic cells—Total Lipid droplets Area	12 h	178.5 ± 14.3	224.95 ±7.4 ***	176.6 ± 2.5 §§§	188.3 ±11.6 §§
	48 h	144.7 ± 31.2	190.1 ± 10.8 *	140.2 ± 5.4 §	154.6 ± 9.0
Steatotic cells—Number of Lipid droplets	12 h	6.99 ± 0.48	6.7 ± 0.04	6.2 ± 0.1	6.5 ± 0.3
	48 h	6.2 ± 1.4	5.7 ± 0.3	5.6 ± 0.1	5.9 ± 0.2
Steatotic cells—Number of Lipid droplets per Area of Cytoplasm	12 h	0.020 ± 0.0006	0.017 ± 0.0002 **	0.019 ± 0.0006 §§	0.019 ± 0.0003 §§
	48 h	0.019 ± 0.0009	0.017 ± 0.0003 *	0.018 ± 0.0003	0.019 ± 0.0003 §§
Steatotic cells—Lipid droplets Area (µm²)	12 h	63.7 ± 5.1	80.4 ± 2.7 ***	63.1 ± 0.9 §§§	67.2 ± 4.1 §§
	48 h	51.7 ± 11.2	67.9 ± 3.8 *	50.1 ± 1.9 §	55.19 ± 3.2
Total cells—Total Lipid droplets Area	12 h	158.75 ± 8.2	217.2 ± 7.2 ****	164.1 ± 4.3 §§§§	172 ± 11.3 §§§§
	48 h	126.3 ± 18.9	177.9 ±13.1 ***	112.8 ± 2.5 §§§	140.0 ± 7.2 §§
Total cells—Number of Lipid droplets	12 h	6.8 ± 0.2	6.52 ± 0.05	6.03 ± 0.1 **	6.2 ± 0.3 *
	48 h	5.7 ± 1.0	5.4 ± 0.3	5.19 ± 0.07	5.6 ± 0.2
Total cells—Number of Lipid droplets per Area of Cytoplasm	12 h	0.02 ± 0.0006	0.018 ± 0.0003 ****	0.02 ± 0.0007 §§	0.02 ± 0.0003 §§§
	48 h	0.019 ± 0.0006	0.017 ± 0.0003 **	0.018 ± 0.0004	0.019 ± 0.0004 §§§
Total cells—Lipids droplets Area (µm²)	12 h	56.5 ± 2.9	77.68 ± 2.6 ****	58.71 ± 1.5 §§§§	61.5 ± 4.0 §§§§
	48 h	45.2 ± 6.7	63.6 ± 4.6 ***	43.9 ± 0.9 §§§	50.1 ± 2.5 §§
Total cells—Cell Volume (µm³)	12 h	3312.87 ± 1036.7	4039.5 ± 2256.5	2299.37 ± 787.3	3741.5 ± 1731
	48 h	1727 ± 528.8	2014.2 ± 986.2	1585.7 ± 433.3	1903.25 ± 794.1
Total cells—Cell Surface Area (µm²)	12 h	3359.3 ± 473.5	4449.8 ± 2846.1	3414.1 ± 834.9	4444.3 ± 2333.8
	48 h	1726.8 ± 285.8	2162.2 ± 1089.7	1753.7 ± 442.3	2261 ± 1239.4

* vs. CTRL (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001); ^§ vs. PA:OA (^§ p < 0.05, ^§§ p < 0.01, ^§§§ p < 0.001, ^§§§§ p < 0.0001).