Figure 3.

KLF15 is reduced in erastin-treated HK2 cells. (A) HK2 cells subjected to erastin (1 μmol/L) were cultured for 12 and 24 h, and cell viability was measured. (B,C). Levels of MDA and GSH in each group at 12 h or 24 h after erastin treatment. (D) The expression of KLF15, GPX4, and SLC7A11 in the indicated cells was tested by Western blotting. GAPDH was used as a loading control. (E) Relative mRNA expression of KLF15, SLC7A11, and GPX4 detected by RT-qPCR in HK2 cells treated with erastin for 12/24 h. KLF15, Krüppel-like factor 15; HK2, human tubular epithelial cells; GSH, glutathione; MDA, malonaldehyde; GPX4, glutathione peroxidase 4. Values are expressed as the mean ± SD. n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, compared to control.