Skip to main content
. 2023 Sep 26;24(19):14530. doi: 10.3390/ijms241914530

Figure 6.

Figure 6

KLF15 post-transcriptionally activates NRF2. (A,B) NRF2 expression was analyzed in KLF15-overexpressing or KLF15 knockdown HK2 cells by Western blotting. (C). NRF2 and GPX4 protein expression was measured in kidney samples of KLF15-knockout mice. (D,E) Western blot analysis of NRF2 in the cytosolic and nuclear fractions of KLF15-overexpressing or KLF15 knockdown in HK2 cells. GAPDH and histone H3 were used as loading controls. (F,G) RT-qPCR was used to detect the expression of NRF2 and its target genes, including GPX4, HO-1, NQO1, and GCLM. (H) NRF2 mRNA expression was measured in kidney samples of KLF15-knockout mice. KLF15, Krüppel-like factor 15; NRF2, nuclear factor erythroid 2-related factor 2; GPX4, glutathione peroxidase 4; HO-1, heme oxygenase-1; NQO1, NADPH quinone oxidoreductase 1; GCLM, glutamate-cysteine ligase modifier subunit. * p < 0.05, ** p < 0.01, **** p < 0.0001.