Table 1.
List of oligonucleotides synthesized using dodecyl-containing phosphoramidites and structures of introduced lipophilic residues. Non-nucleotide and fluorescence units in the oligonucleotide sequence which were incorporated using the corresponding phosphoramidites or CPG: [Dcyl]—5′-terminal dodecyl moiety; [X]—dodecyl unit; [Db]—doubler; [Tb]—trebler; [FAM]—5′-fluorescein moiety; [FAM’]—3′-fluorescein moiety.
| Code | Sequence (5′–3′) | MS (Calc/Found) |
|---|---|---|
| O1 | [Dcyl]-CTG-ACT-ATG-AAG-TATT-[FAM’] | 5794.1/5796.0 |
| O2 | [Dcyl]2[Db]-CTG-ACT-ATG-AAG-TATT-[FAM’] | 6394.7/6392.4 |
| O3 | [Dcyl]3[Tb]-CTG-ACT-ATG-AAG-TATT-[FAM’] | 6721.1/6722.8 |
| N3 | [FAM]-CTG-ACT-ATG-AAG-TAT-[X][X][X]-T | 6706.0/6705.6 |
| C | [FAM]-CTG-ACT-ATG-AAG-TATT | -/- |
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