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. 2023 Sep 22;20(19):6806. doi: 10.3390/ijerph20196806

Table 1.

SARS-CoV-2 RNA results of the 60 surface samples taken on 24 and 25 June 2021 from the meat processing plant following an outbreak.

RT-PCR Results
(From a Total of 60 Samples)
Level of RNA
(Based on Ct Value)
Confirm
Positive
Suspected Positive Negative Moderate-High (Ct < 32.0) Low (Ct 32.0–34.9) Very Low-None (Ct ≥ 35.0 a)
1 (1.7%) 6 (10.0%) 53 (88.3%) 0 (0.0%) 1 (1.7%) 59 (98.3%)
Positive sample information
Site area Location in area Mean Ct value b Estimated copies per cm2 c
Engineering Toolbox cupboard-door and 2 shelves 34.0 1007
Engineering Toolbox cupboard-top of cupboard 37.9 d 40
Engineering Tool drawers 36.5 d 240
Chiller 1 OCM screen 37.9 d 14
Mission control Dehumidifier 38.0 d 34
Smoking
shelter
Chair 37.5 d 102
Canteen Table top and seat 37.7 d 38

a Includes 53 samples with no SARS-CoV-2 RNA detected. b Mean Ct value for the N gene. c Extrapolation from copies per reaction to copies per sample collected based on the dilution factor, then divided by recorded sampling area. d Sample identified as suspected positive, defined as a sample with a single replicate testing positive for at least one target. The detection limit defined by the manufacturer was Ct 38.0. Abbreviations: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Ribonucleic acid (RNA), Real-time polymerase chain reaction (RT-PCR), Crossing threshold (Ct) and Nucleocapsid (N).