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. 2023 Sep 28;5(1):vdad117. doi: 10.1093/noajnl/vdad117

Figure 5.

Figure 5.

Exploration of the antitumor mechanism of YSCH-01. (A) Flow cytometry was used to detect the apoptosis of LN-18 tumor cells after treatment. (B) Under 10 MOI of virus treatments, the comparison of PI proportion among Annexin V-FITC-positive LN-18 cells. (C) Flow cytometry was used to detect the apoptosis of LN-229 tumor cells after treatment. (D) Under 10 MOI of virus treatments, the comparison of PI proportion among Annexin V-FITC-positive LN-229 cells. (E) Western Blot to detect makers related to cell apoptosis. (F) The representative immuno-histochemical staining images of CD31, Ki67, CD45 in U-118 MG xenograft tumor sections 13 days after drug treatments. Scale bar, 100 μm. (G) The representative TUNEL immunofluorescent staining images of U-118 MG xenograft tumor sections 13 days after drug treatments. Scale bar, 1 mm. n.s., not significant, *P < .05, **P < .01, ***P < . 001, ****P < . 0001, one-way ANOVA followed by Tukey’s multiple comparisons test.