TABLE 3.
Summary of mutations on the B. subtilis rpsL gene resulting in amino acid exchange in the ribosomal protein S12
| B. subtilis strain | Amt of antibiotic produced (U/ml)a | Spores/mlb in sporulation medium
|
Position of mutation in rpsL genec | Amino acid position (exchange) | Resistance to streptomycin (μg/ml)d | |
|---|---|---|---|---|---|---|
| Alone | Plus 0.5% glucose | |||||
| 168 (parent strain) | 8 | 1.5 × 109 | 2.5 × 108 | 1 | ||
| 60009 (strA1) | 6 | 3 × 108 | 5 × 107 | A-167→T | 56 (Lys→Ile) | 1,000 |
| KO-263 (str-1) | 8 | 4 × 108 | 2 × 107 | A-168→T | 56 (Lys→Asn) | 500 |
| KO-264 (str-2) | 6 | 6 × 108 | 3 × 108 | A-168→T | 56 (Lys→Asn) | 500 |
| KO-265 (str-3) | 6 | 8 × 108 | 3 × 108 | A-168→T | 56 (Lys→Asn) | 500 |
| KO-266 (str-4) | 6 | 9 × 108 | 2 × 108 | A-168→T | 56 (Lys→Asn) | 500 |
| KO-267 (str-5) | 64 | 1.2 × 109 | 1 × 108 | A-167→G | 56 (Lys→Arg) | 2,000 |
| KO-268 (str-6) | 64 | 1.5 × 109 | 3 × 107 | A-167→G | 56 (Lys→Arg) | 1,000 |
| KO-269 (str-7) | 80 | 1 × 109 | 4 × 107 | A-167→G | 56 (Lys→Arg) | 1,000 |
| KO-270 (str-8)e | 80 | 8 × 108 | 1 × 108 | C-64→T | 22 (Pro→Ser) | 1,000 |
| A-167→G | 56 (Lys→Arg) | |||||
| KO-271 (str-9) | 80 | 1.5 × 109 | 1 × 107 | A-167→C | 56 (Lys→Thr) | 1,000 |
| KO-272 (str-10) | 42 | 1 × 108 | 1 × 107 | A-166→C | 56 (Lys→Gln) | 400 |
| KO-273 (str-11) | 30 | 1 × 108 | 1 × 107 | A-166→C | 56 (Lys→Gln) | 400 |
| KO-274 (str-12) | 380 | 3 × 109 | 3 × 109 | Nonef | 20 | |
| KO-275 (str-13) | 240 | 2.5 × 109 | 1.5 × 109 | None | 5 | |
| KO-276 (str-14) | 80 | 8 × 108 | 1 × 108 | None | 20 | |
| KO-277 (str-15) | 64 | 2 × 109 | 2 × 108 | None | 5 | |
| KO-278 (str-16) | 80 | 8 × 108 | 3 × 108 | None | 5 | |
a
Determined after 2 days of culture at 30°C in NG medium.
b
Determined after 20 h of culture at 37°C in sporulation medium with or without glucose as indicated.
c
Numbering originates from the start codon (ATG) of the open reading frame.
d
Determined after 24 h of incubation at 37°C on NG agar.
e
This mutant revealed double mutation (C-64 and A-167) within the rpsL gene.
f
Mutations were not detected within the rpsL gene.