Figure 1.

Glypican 1 knockout mice show a modified glycocalyx. (A) Representative electron micrographs of WT and Gpc1^−/− showing endothelial glycocalyx (left panel), areas selected in the red square are shown at higher magnification on the right panel with prominent endothelial glycocalyx cluster-like pattern (red arrowheads). In the plot, Gpc1^−/− showed increased endothelial glycocalyx thickness when compared to WT. Scale bar: 0.5 µm. N = 190 measurements for WT and N = 230 measurements for Gpc1^−/−. (B) Gpc1^−/− showed decreased expression of syndecan 4 (Sdc4) but not of syndecan 1 (Sdc1) in lung tissue when compared to WT; p values resulting from unpaired Student’s t test are shown for each comparison. * Indicates statistical significance (p < 0.05) vs. WT, unpaired t test, N ≥ 5/group. Representative bands from different gels or not in consecutive sequence are separated by dashed lines. Brightness and contrast adjustments were applied equally to all images for representative images. M: molecular weight ladder.