Figure 3.

Parental folate deficiency increased Caspase-3 expression in neural cells in neonatal offspring rats’ hippocampus and cerebral cortex. Offspring rats were grouped as described in Figure 1. (a) Representative images of Caspase-3 expression in neural cells in the hippocampus and cerebral cortex, in which cells are stained red with Caspase-3 and blue with DAPI. Scale bar = 50 μm. Each column below the merged image shows an enlarged image of the corresponding image rectangle on the merged image. (b) Caspase-3 expression in neural cells in the hippocampus. (c) Caspase-3 expression in neural cells in the cerebral cortex. Data are expressed as mean ± SD (n = 5). *: p < 0.05.