Figure 8.

HAMNO fails to radiosensitize A549 cells. (A) Exponentially growing cells were treated with HAMNO 30 min before irradiation. Cells were then allowed to repair for 16 h and plated in the absence of HAMNO. (B) Cells were enriched in the S-phase by treating them with the CDK4/6 selective inhibitor PD-0332991 followed by a single thymidine block. After release in fresh medium, cells were exposed to IR, allowed to repair for 4 h and plated in the absence of HAMNO. ns (not significant) as determined by Mann–Whitney U tests.