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. 2023 Oct 9;24(19):15032. doi: 10.3390/ijms241915032

Table 1.

Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis of the gene expression in human coronary artery endothelial cells (HCAECs) and human internal thoracic artery endothelial cells (HITAECs) cultured at laminar flow or in static conditions. Expression has been normalised for the expression of three housekeeping genes (GAPDH, ACTB, and B2M).

Gene Flow Culture Chambers
(µ-Slide y-Shaped)		 Static Conditions
Experiment #1		 Static Conditions
Experiment #2		 Static Conditions
Experiment #3		 Static Conditions
Experiment #4
HCAECs, ΔCt
(Mean ± Standard Deviation)		 HITAECs, ΔCt
(Mean ± Standard Deviation)		 Fold Change (HCAECs to
HITAECs)		 HCAECs, ΔCt
(Mean ± Standard Deviation)		 HITAECs, ΔCt
(Mean ± Standard Deviation)		 Fold Change (HCAECs to
HITAECs)		 HCAECs, ΔCt
(Mean ± Standard Deviation)		 HITAECs, ΔCt
(Mean ± Standard Deviation)		 Fold Change (HCAECs to
HITAECs)		 HCAECs, ΔCt
(Mean ± Standard Deviation)		 HITAECs, ΔCt
(Mean ± Standard Deviation)		 Fold Change (HCAECs to
HITAECs)		 HCAECs, ΔCt
(Mean ± Standard Deviation)		 HITAECs, ΔCt
(Mean ± Standard Deviation)		 Fold Change (HCAECs to
HITAECs)
VCAM1 0.0051 ± 0.0004 0.0053 ± 0.0006 0.962 0.0015 ± 0.0004 0.0098 ± 0.0009 0.153 N/D N/D N/D 0.0013 ± 0.0002 0.0955 ± 0.0039 0.014 0.0051 ± 0.0002 0.0455 ± 0.0041 0.112
ICAM1 0.0331 ± 0.0021 0.1659 ± 0.0121 0.200 0.0050 ± 0.0003 0.0407 ± 0.0027 0.123 0.0279 ± 0.0132 0.0559 ± 0.0231 0.499 0.0163 ± 0.0079 2.0781 ± 0.7478 0.008 0.0313 ± 0.0096 0.2675 ± 0.1376 0.117
SELE 0.0012 ± 0.0001 0.0265 ± 0.0067 0.045 0.0011 ± 0.0002 0.0481 ± 0.0039 0.023 0.0002 ± 0.00002 0.0219 ± 0.0016 0.009 0.0004 ± 0.0001 0.0942 ± 0.0110 0.004 0.0029 ± 0.0002 0.0939 ± 0.0057 0.031
SELP 0.0010 ± 0.0001 0.0184 ± 0.0028 0.054 0.0025 ± 0.0009 0.0044 ± 0.0011 0.568 0.0899 ± 0.0035 0.0080 ± 0.0037 11.238 0.0127 ± 0.0016 0.0555 ± 0.0099 0.229 0.0321 ± 0.0022 0.0203 ± 0.0011 1.581
IL6 0.0044 ± 0.0004 0.0199 ± 0.0016 0.221 0.0029 ± 0.0007 0.0007 ± 0.0003 4.143 0.0042 ± 0.0003 0.0211 ± 0.0019 0.199 0.0029 ± 0.0002 0.0095 ± 0.0020 0.305 0.0084 ± 0.0009 0.0050 ± 0.0003 1.680
CXCL8 0.0337 ± 0.0009 0.1774 ± 0.0129 0.190 0.0701 ± 0.0045 0.0557 ± 0.0051 1.259 0.0256 ± 0.0029 0.3286 ± 0.0285 0.078 0.0301 ± 0.0028 0.5886 ± 0.0732 0.051 0.1107 ± 0.0063 0.4469 ± 0.0335 0.248
CCL2 0.0112 ± 0.0009 0.2993 ± 0.0247 0.037 0.0279 ± 0.0091 0.2563 ± 0.0137 0.109 0.0614 ± 0.0038 1.0850 ± 0.1059 0.057 0.0543 ± 0.0026 4.3406 ± 0.9546 0.013 0.0920 ± 0.0048 0.9923 ± 0.0732 0.093
CXCL1 N/D N/D N/D 0.0763 ± 0.0080 0.0651 ± 0.0061 1.172 0.0984 ± 0.0274 0.2571 ± 0.0556 0.383 0.1074 ± 0.0154 1.6440 ± 0.4153 0.065 0.2617 ± 0.0185 0.2715 ± 0.0439 0.964
MIF N/D N/D N/D 0.2152 ± 0.0231 0.0812 ± 0.0074 2.650 0.4353 ± 0.0956 2.6439 ± 0.1977 0.165 0.1903 ± 0.0117 4.0451 ± 0.5167 0.047 0.4234 ± 0.0139 0.6183 ± 0.0129 0.685
KLF2 0.0047 ± 0.0006 0.0021 ± 0.0002 2.238 0.0018 ± 0.0009 0.0004 ± 0.0001 4.500 N/D N/D N/D N/D N/D N/D N/D N/D N/D
KLF4 0.0239 ± 0.0012 0.1268 ± 0.0178 0.188 0.0010 ± 0.0002 0.0005 ± 0.0001 2.000 N/D N/D N/D N/D N/D N/D N/D N/D N/D
NFE2L2 0.0618 ± 0.0041 0.0293 ± 0.0010 2.109 0.0866 ± 0.088 0.0523 ± 0.0026 1.656 N/D N/D N/D N/D N/D N/D N/D N/D N/D
SNAI1 0.0312 ± 0.0011 0.3193 ± 0.0632 0.098 0.0071 ± 0.0004 0.0121 ± 0.0017 0.587 0.0286 ± 0.0031 0.0381 ± 0.0065 0.751 0.0011 ± 0.0001 0.1209 ± 0.0140 0.009 0.0236 ± 0.0011 0.0237 ± 0.0031 0.996
SNAI2 0.0003 ± 0.00002 0.0002 ± 0.00004 1.500 0.0002 ± 0.00001 0.0001 ± 0.00001 2.000 0.0371 ± 0.0045 0.0025 ± 0.0007 14.840 0.00006 ± 0.00002 0.0043 ± 0.0015 0.014 0.0034 ± 0.0002 0.0001 ± 0.00005 34.000
TWIST1 0.0005 ± 0.0001 0.0001 ± 0.00001 5.000 0.0004 ± 0.00001 0.00001 ± 0.00001 40.000 0.0010 ± 0.0004 0.0007 ± 0.0002 1.429 0.00008 ± 0.00002 0.00014 ± 0.0001 0.571 0.0002 ± 0.00004 0.00004 ± 0.00002 5.000
ZEB1 0.0845 ± 0.0096 0.0886 ± 0.0056 0.954 0.0350 ± 0.0057 0.0343 ± 0.0034 1.020 0.0818 ± 0.0037 0.1628 ± 0.0196 0.502 0.0068 ± 0.0004 0.0655 ± 0.0094 0.104 0.0486 ± 0.0024 0.0552 ± 0.0083 0.880
CDH5 0.6792 ± 0.0132 3.0981 ± 0.3207 0.219 0.4247 ± 0.0985 0.5115 ± 0.0189 0.830 0.3888 ± 0.0087 0.3852 ± 0.0165 1.009 0.9632 ± 0.0871 9.4160 ± 2.8673 0.102 1.1429 ± 0.1359 2.1643 ± 0.0577 0.528
CDH2 0.0088 ± 0.0013 0.0103 ± 0.0015 0.854 0.0108 ± 0.0015 0.0028 ± 0.0009 3.857 0.0670 ± 0.0056 0.0001 ± 0.0002 670.000 0.0479 ± 0.0032 0.0146 ± 0.0039 3.281 0.1393 ± 0.0119 0.0079 ± 0.0003 17.633
NOS3 0.0450 ± 0.0008 0.1536 ± 0.0102 0.293 0.0066 ± 0.00011 0.0020 ± 0.0007 3.300 0.0015 ± 0.0004 0.0009 ± 0.0007 1.667 0.0057 ± 0.0005 0.2375 ± 0.0387 0.024 0.0262 ± 0.0026 0.0125 ± 0.0003 2.096

N/D—not defined (have not been measured).