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. 2023 Sep;29(9):1411–1422. doi: 10.1261/rna.079678.123

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© 2023 Li et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 5. — The effect of a bulged adenine inserted between G1b and A2n on the folding of the Arabidopsis and DUF-3268 k-junctions. The fluorescent constructs of the Arabidopsis and DUF-3268 k-junctions were modified so that the bulged adenine was deleted from the former, and an adenine was inserted between G1b and A2n in the latter. All four species were titrated with Mg²⁺ ions. E_FRET has been plotted against Mg²⁺ ion concentration for the Arabidopsis k-junction unmodified (Arab; filled squares) and with the bulged A deleted (Arab ΔA; open squares), and for the DUF-3268 k-junction unmodified (DUF; filled circles) and with the inserted adenine (DUF +A; open circles). The data are fitted to a simple two-state binding model (lines).