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. 2023 Oct 3;24(19):14865. doi: 10.3390/ijms241914865

Figure 4.

Figure 4

Activation of target gene expression by CRISPRa tools. (A,B) The dCas9 protein recruits the VP64 domain (A) or VPR activation system (B) to the promoter region of the target gene and acts on the promoter to activate the expression of the target gene. (C) MCP-p65-HSF1 complex recruited by MS2 co-activates the target gene expression with VP64 effector recruited by dCas9 protein. (D,E) dCas9 recruits the p300 core domain to the promoter or enhancer of the target gene to catalyze H3K27ac in this region, resulting in activation of gene expression. (F) dCas9 recruits the dMSK1 domain to the promoter of the target gene to catalyze the production of H3S28ph in this region, inducing activation of target gene expression.