Table 1.

Summary of pulses parameters in examined exposure protocols and measured temperature increase (ΔT). PRR = pulse repetition rate (Hz); NA = not applicable, as the temperature increase was insignificant (below 1 °C). Number of independent experiments (N) in ZAP was equal to 1. For all experiments number of biological replicates (n) was ≥6.

	Pulse Duration d (ns)	Number of Pulses N	Cumulative Time Duration D (μs)	Total Exposure Duration t (s)	Absorbed Energy Density u (mJ/mm3) in HBSS	Temperature Increase-ΔT (°C) in HBSS	Absorbed Energy Density u (mJ/mm3) in ZAP	Temperature Increase-ΔT (°C) in ZAP	E-Field Intensity in HBSS (kV/cm)	E-field Intensity in ZAP (kV/cm)	Number of Independent Experiments HBSS (N)
Protocol 1
(PRR = 20 Hz)	2	500	1	25	140	3.8 ± 0.2	16	NA	100	90	3
	4	500	2	25	280	5.7 ± 0.3	32	NA	100	90	3
	6	500	3	25	420	9.1 ± 1.8	47	NA	100	90	3
	8	500	4	25	560	10.8 ± 2.2	65	NA	100	90	3
	10	500	5	25	700	15.1± 2.7	81	1.1 ± 0.2	100	90	3
Protocol 2
(PRR = 200 Hz)	2	500	1	2.5	140	12.6 ± 0.8	16	1.2 ± 0.1	100	90	2
	6	500	3	2.5	420	32.2 ± 0.7	47	2.5 ± 0.1	100	90	2
	10	500	5	2.5	700	46.8 ± 3.9	81	4.4 ± 0.2	100	90	2
Protocol 3
(PRR = 20 Hz)	2	2500	5	125	700	8.5 ± 1.5	81	NA	100	90	3
	6	833	5	41.6	700	15.2 ± 1.9	81	NA	100	90	3
	10	500	5	25	700	16.8 ± 2.2	81	1.1 ± 0.2	100	90	3
Protocol 4
(PRR = 200 Hz)	2	2500	5	12.5	700	22.0 ± 2.2	81	3.2 ± 0.1	100	90	2
	6	833	5	4.16	700	42.8 ± 1.4	81	3.7 ± 0.4	100	90	2
	10	500	5	2.5	700	46.8 ± 3.9	81	4.4 ± 0.2	100	90	2