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. 2023 Sep 30;24(19):14808. doi: 10.3390/ijms241914808

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Treatment with the hexokinase inhibitor 2-DG attenuated the proliferation and migration of Hep2 cells induced by intermittent hypoxia-exposed M2-type macrophage supernatant. Hep2 cells were cultured in intermittent hypoxia-exposed M2-type macrophage supernatant and treated with 2-DG or solvent control, and the following assays were performed. (A) Colony formation assay. (B) Transwell migration assay (100×). (C,D) Lactate assay. (E,F) Pyruvate assay. The data are presented as the mean ± SD values. * p < 0.05; ** p < 0.01; *** p < 0.001.