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. 2023 Sep 30;24(19):14808. doi: 10.3390/ijms241914808

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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ZBTB10 binds to a candidate promoter sequence in HK1 and transcriptionally regulates HK1 gene expression. (A) Representative immunoblot analysis of HK1, ZBTB10 and HA protein expression in ZBTB10-overexpressing cells. (B) Representative immunoblot analysis of HK1 and ZBTB10 protein expression in ZBTB10 knockout cells. (C) Luciferase activity of HK1 in ZBTB10-overexpressing 293T cells. (D) Luciferase activity of HK1 in ZBTB10 knockout 293T cells. (E,F) Luciferase activity of HK1 in ZBTB10-overexpressing and ZBTB10 knockout Hep2 cells. (G) Fold enrichment of the positive control RPL30 in the ChIP assay. (H) Fold enrichment of HK1 in the ChIP assay. * p < 0.05; ** p < 0.01; *** p < 0.001.