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. 2023 Oct 9;24(19):15018. doi: 10.3390/ijms241915018

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Pluripotency marker and epigenetic regulatory expression in hFM-MSCs upon BPs and/or PFs treatment. The gene expression of the stemness identifiers (NANOG, OCT4, SOX2, KLF4, ESG1, and OVOL1) and epigenetic regulators (DNMT1, DNTM3A, TET1, TET2, and TET3) were detected by qPCR in hFM-MSCs treated with BPs and/or PFs for 24 h, as indicated. The fold changes were determined from the −ΔΔCt values calculated using 18S as a reference gene and normalized to untreated hFM-MSCs as control condition (CTRL). The graphs show the mean ± SD of 3 independent experiments, * p < 0.05 vs. CTRL. Bisphenol A (BPA), Bisphenol S (BPS), perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), BPA + PFOS, BPS + PFOA, BPS + PFOS, BPS + PFOA (ALL).