Table 3.
This table includes the primer sequence for qPCR.
| Gene | Sequence (5′ to 3′) | Reference |
|---|---|---|
| NANOG_FW | CCAGACCCAGAACATCCAGTC | [17] |
| NANOG_REV | CACTGGCAGGAGAATTTGGC | |
| OCT4_FW | GGGTTTTTGGGATTAAGTTCTTC | [56] |
| OCT4_REV | GCCCCCACCCTTTGTGTT | |
| SOX2_FW | CAAAAATGGCCATGCAGGTT | [56] |
| SOX2_REV | AGTTGGGATCGAACAAAAGCTATT | |
| ESG1_FW | CCATGAATGCCCTCGAACTAGG | [19] |
| ESG1_REV | CCTTAACTCTTTAGGCTGGAGCA | |
| KLF4_FW | AGCCTAAATGATGGTGCTTGGT | [56] |
| KLF4_REV | TTGAAAACTTTGGCTTCCTTGTT | |
| OVOL1_FW | AGAGCAGAGACCATGGCTTC | [50] |
| OVOL1_REV | GACGTGTCTCTTGAGGTCGA | |
| DNMT1_FW | GCCAGAGATAGAGATCAAGCTG | Primer blast |
| DNMT1_REV | CACAGCGTGTCAGAGATGCC | |
| DNTM3A_FW | CCATCGTCAACCCTGCTCG | Primer blast |
| DNMT3A_REV | CACCACATTCTCAAAGAGCCAG | |
| DNTM3B_FW | GACTCGTTCAGAAAGCCCAG | Primer blast |
| DNMT3B_REV | GGACTCGTCCACATGGTTGC | |
| TET1_FW | ACTCCCTGAGGTCTGTCCTG | Primer blast |
| TET1_REV | CAGGTAGGGCTGCATGACTT | |
| TET2_FW | CTCAGCAGCAGCCAATAGGA | Primer blast |
| TET2_REV | CTGTCTGGCAAATGGGAGGT | |
| TET3_FW | AACTGCTCACTCAGCTCTGC | Primer blast |
| TET3_REV | GCAGCCCTCAGAAAAGGGAT | |
| 18S_FW | CATGGCCGTTCTTAGTTGGT | [57] |
| 18S_REV | CGCTGAGCCAGTCAGTGTAG |